ELISA試劑盒的應注意防護

<p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">是固相夾心法酶聯(lián)免疫吸附實驗（</font><font face="Calibri">ELISA</font><font face="宋體">）</font><font face="Calibri">.</font><font face="宋體">已知濃度的標準品、未知濃度的樣品加入微孔酶標板內(nèi)進行檢測。先將生物素標記的抗體同時溫育。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">洗滌后，</font><strong>ELISA<font face="宋體">試劑盒</font></strong><font face="宋體">加入親和素標記過的</font><font face="Calibri">HRP</font><font face="宋體">。再經(jīng)過溫育和洗滌，去除未結(jié)合的酶結(jié)合物，然后加入底物</font><font face="Calibri">A</font><font face="宋體">、</font><font face="Calibri">B</font><font face="宋體">，和酶結(jié)合物同時作用。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">產(chǎn)生顏色：</font>ELISA<font face="宋體">試劑盒顏色的深淺和樣品中的濃度呈比例關(guān)系。</font><font face="Calibri">ELSIA</font><font face="宋體">操作步驟復雜，影響反應因素較多，特別是固相載體的包被難達到各個體之間的一致</font><font face="Calibri">.</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">因此在定量測定中，</font> ELISA<font face="宋體">試劑盒每批測試均須用一系列不同濃度的參考標準品在相同的條件下制作標準曲線。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">測定大分子量物質(zhì)的夾心法</font>ELISA<font face="宋體">試劑盒，標準曲線的范圍一般較寬，曲線最高點的吸光度可接近</font><font face="Calibri">2.0</font><font face="宋體">，繪制時常用半對數(shù)紙，以檢測物的濃度為橫坐標，</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒以吸光度為縱坐標，將各濃度的值逐點連接，所得曲線一般呈</font><font face="Calibri">S</font><font face="宋體">形，其頭、尾部曲線趨于平坦，中央較呈直線的部分是最理想的檢測區(qū)域。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">酶的底物及供氫體的選擇：對供氫體的選擇要求是價廉、安全、有明顯地顯色反應，而本身無色。有些供氫體（如</font><font face="Calibri">OPD</font><font face="宋體">等）有潛在的致癌作用。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">有條件者應使用不致癌、靈敏度高的供氫體，如</font>TMB<font face="宋體">和</font><font face="Calibri">ABTS</font><font face="宋體">是目前較為滿意的供氫體。</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒底物作用一段時間后，應加入強酸或強堿以終止反應。通常底物作用時間，以</font><font face="Calibri">10-30</font><font face="宋體">分鐘為宜。底物使用液必須新鮮配制，尤其是</font><font face="Calibri">H2O2</font><font face="宋體">在臨用前加入。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>